zetaprobe gt membrane (Bio-Rad)
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zetaprobe gt membrane
Zetaprobe Gt Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1714 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zetaprobe+gt+membranes/Zeta-Probe+GT+Membrane/pm41418758-435-6-9
Average 96 stars, based on 1714 article reviews
Zetaprobe Gt Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1714 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zetaprobe+gt+membranes/Zeta-Probe+GT+Membrane/pm41418758-435-6-9
Average 96 stars, based on 1714 article reviews
zetaprobe gt membrane - by Bioz Stars,
2026-10
96/100 stars
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BAC Assay:Article Title: Vein patterning screens and the <i>defectively organized tributaries</i> mutants in <i>Arabidopsis thaliana</i> Article Snippet: .. TAC filters (Liu et al., 1995) were probed with 1-kb genomic probes spaced 20–40-kb apart, based on BAC sequences, according to the manufacturer’s instructions provided for Southern Blot:Article Title: Fork-barrier-independent roles of topoisomerase I in the ribosomal DNA. Article Snippet: Topoisomerase I, a nickase that allows swiveling of the DNA substrate, is highly enriched in the ribosomal DNA (rDNA) from yeast to humans, but its function at this locus remains poorly understood.. S. cerevisiae mutants lacking topoisomerase I (top1) exhibit pronounced rDNA instability and accumulate bubbles of single-stranded DNA (ssDNA) on 35S ribosomal RNA genes, suggesting a role in relieving transcription-associated topological stress.. However, Top1 cleavage complexes are most highly enriched in the rDNA-encoded replication fork barrier, a genetically encoded source of rDNA instability, and only weakly in the 35S promoter, leading to the proposal that top1-associated rDNA instability may be linked to the fork barrier. Article Title: Topoisomerase I promotes formation of a novel DNA intermediate in the ribosomal DNA Article Snippet: Plugs were embedded in 1% SeaKem LE agarose and rDNA fragments separated in 0.5X TBE in a BioRad CHEF-DR II instrument at settings: 5.1 V/cm, 23 h, 5-20 s switch-time ramp (for short rDNA fragments) or 6 V/cm, 60 s switch time for 15 h, followed by 90 s switch time for 9 hours (for large arrays). .. We performed Southern blotting using alkaline transfer onto Hybond-XL (Cytiva) or Mutagenesis:Article Title: The Subcellular Distribution of an RNA Quality Control Protein, the Ro Autoantigen, Is Regulated by Noncoding Y RNA Binding Article Snippet: .. Other antibodies were mouse anti-FLAG M2 (Sigma-Aldrich), mouse anti-actin (Millipore Bioscience Research Reagents, Temecula, CA) and monoclonal anti-Sm (Y12; a gift of J. Steitz, Yale University, New Haven, CT). fig ft0 fig mode=article f1 Figure 1. caption a4 A mutant Ro protein that does not bind Y RNAs accumulates in nuclei. (A) Lysates from wild-type (lane 2), Ro −/− (lane 3) and Ro −/− cell lines stably expressing either FLAG 3 -Ro (lane 1) or FLAG 3 -Ro containing the indicated ... Northern Blots RNAs were fractionated in 5% polyacrylamide/8 M urea gels and transferred to Stable Transfection:Article Title: The Subcellular Distribution of an RNA Quality Control Protein, the Ro Autoantigen, Is Regulated by Noncoding Y RNA Binding Article Snippet: .. Other antibodies were mouse anti-FLAG M2 (Sigma-Aldrich), mouse anti-actin (Millipore Bioscience Research Reagents, Temecula, CA) and monoclonal anti-Sm (Y12; a gift of J. Steitz, Yale University, New Haven, CT). fig ft0 fig mode=article f1 Figure 1. caption a4 A mutant Ro protein that does not bind Y RNAs accumulates in nuclei. (A) Lysates from wild-type (lane 2), Ro −/− (lane 3) and Ro −/− cell lines stably expressing either FLAG 3 -Ro (lane 1) or FLAG 3 -Ro containing the indicated ... Northern Blots RNAs were fractionated in 5% polyacrylamide/8 M urea gels and transferred to Expressing:Article Title: The Subcellular Distribution of an RNA Quality Control Protein, the Ro Autoantigen, Is Regulated by Noncoding Y RNA Binding Article Snippet: .. Other antibodies were mouse anti-FLAG M2 (Sigma-Aldrich), mouse anti-actin (Millipore Bioscience Research Reagents, Temecula, CA) and monoclonal anti-Sm (Y12; a gift of J. Steitz, Yale University, New Haven, CT). fig ft0 fig mode=article f1 Figure 1. caption a4 A mutant Ro protein that does not bind Y RNAs accumulates in nuclei. (A) Lysates from wild-type (lane 2), Ro −/− (lane 3) and Ro −/− cell lines stably expressing either FLAG 3 -Ro (lane 1) or FLAG 3 -Ro containing the indicated ... Northern Blots RNAs were fractionated in 5% polyacrylamide/8 M urea gels and transferred to Northern Blot:Article Title: The Subcellular Distribution of an RNA Quality Control Protein, the Ro Autoantigen, Is Regulated by Noncoding Y RNA Binding Article Snippet: .. Other antibodies were mouse anti-FLAG M2 (Sigma-Aldrich), mouse anti-actin (Millipore Bioscience Research Reagents, Temecula, CA) and monoclonal anti-Sm (Y12; a gift of J. Steitz, Yale University, New Haven, CT). fig ft0 fig mode=article f1 Figure 1. caption a4 A mutant Ro protein that does not bind Y RNAs accumulates in nuclei. (A) Lysates from wild-type (lane 2), Ro −/− (lane 3) and Ro −/− cell lines stably expressing either FLAG 3 -Ro (lane 1) or FLAG 3 -Ro containing the indicated ... Northern Blots RNAs were fractionated in 5% polyacrylamide/8 M urea gels and transferred to Article Title: The zipcode-binding protein ZBP1 influences the subcellular location of the Ro 60-kDa autoantigen and the noncoding Y3 RNA Article Snippet: .. For ZBP1, at 48 h after transfection, cells were subjected to a second round of transfection and incubated for an additional 24 h. For Northern analyses, RNAs were separated in 5% polyacrylamide/8 M urea gels and transferred to Hybond-N (GE Healthcare) or Transfection:Article Title: The zipcode-binding protein ZBP1 influences the subcellular location of the Ro 60-kDa autoantigen and the noncoding Y3 RNA Article Snippet: .. For ZBP1, at 48 h after transfection, cells were subjected to a second round of transfection and incubated for an additional 24 h. For Northern analyses, RNAs were separated in 5% polyacrylamide/8 M urea gels and transferred to Hybond-N (GE Healthcare) or Incubation:Article Title: The zipcode-binding protein ZBP1 influences the subcellular location of the Ro 60-kDa autoantigen and the noncoding Y3 RNA Article Snippet: .. For ZBP1, at 48 h after transfection, cells were subjected to a second round of transfection and incubated for an additional 24 h. For Northern analyses, RNAs were separated in 5% polyacrylamide/8 M urea gels and transferred to Hybond-N (GE Healthcare) or |